based genetic algorithm objective tool box Search Results


99
Transnetyx taq man based rtpcr probes
Taq Man Based Rtpcr Probes, supplied by Transnetyx, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Gene Tools Inc sequencebased reagent standard control morpholino
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Ebiogen Inc gene analysis exdega tool
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Gene Tools Inc 5-base c1qr mismatch mo (gtcagtctgatagtactcggtttag)
Clec14a and <t>C1qr</t> have a partially redundant function during angiogenic sprouting. Kdrl: GFP expression in the trunk region imaged by fluorescent microscopy at 28 hpf ( a - d ), 48 hpf ( e - h ) and 72 hpf ( i - l ). a , e , i Wild-type control embryos; b , f , j clec14a−/− mutants; c , g , k Wild-type embryos injected with 10 ng of c1qr MO; d , h , l clec14a−/− mutant embryos injected with 10 ng of c1qr MO. Note the delayed intersegmental vessel sprouting in clec14a mutants ( b ) and c1qr MO embryos ( c ) at 24 hpf (arrowheads). Partial sprouts (arrowheads) and abnormal vascular connections (arrows) are apparent in the clec14a−/−; c1qr MO embryos ( d , h , l ). m Percentage of embryos with vascular defects. ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from 3 independent experiments. Error bars show standard error. n Distribution of different types of phenotypes in embryos at 48 hpf. Data were combined from two independent experiments
5 Base C1qr Mismatch Mo (Gtcagtctgatagtactcggtttag), supplied by Gene Tools Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Gene Tools Inc sense control morpholino oligonucleotide (co-mo) composed of base sequence 5′-ccagaatgctttccaaactaatcca-3′
Clec14a and <t>C1qr</t> have a partially redundant function during angiogenic sprouting. Kdrl: GFP expression in the trunk region imaged by fluorescent microscopy at 28 hpf ( a - d ), 48 hpf ( e - h ) and 72 hpf ( i - l ). a , e , i Wild-type control embryos; b , f , j clec14a−/− mutants; c , g , k Wild-type embryos injected with 10 ng of c1qr MO; d , h , l clec14a−/− mutant embryos injected with 10 ng of c1qr MO. Note the delayed intersegmental vessel sprouting in clec14a mutants ( b ) and c1qr MO embryos ( c ) at 24 hpf (arrowheads). Partial sprouts (arrowheads) and abnormal vascular connections (arrows) are apparent in the clec14a−/−; c1qr MO embryos ( d , h , l ). m Percentage of embryos with vascular defects. ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from 3 independent experiments. Error bars show standard error. n Distribution of different types of phenotypes in embryos at 48 hpf. Data were combined from two independent experiments
Sense Control Morpholino Oligonucleotide (Co Mo) Composed Of Base Sequence 5′ Ccagaatgctttccaaactaatcca 3′, supplied by Gene Tools Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Clec14a and C1qr have a partially redundant function during angiogenic sprouting. Kdrl: GFP expression in the trunk region imaged by fluorescent microscopy at 28 hpf ( a - d ), 48 hpf ( e - h ) and 72 hpf ( i - l ). a , e , i Wild-type control embryos; b , f , j clec14a−/− mutants; c , g , k Wild-type embryos injected with 10 ng of c1qr MO; d , h , l clec14a−/− mutant embryos injected with 10 ng of c1qr MO. Note the delayed intersegmental vessel sprouting in clec14a mutants ( b ) and c1qr MO embryos ( c ) at 24 hpf (arrowheads). Partial sprouts (arrowheads) and abnormal vascular connections (arrows) are apparent in the clec14a−/−; c1qr MO embryos ( d , h , l ). m Percentage of embryos with vascular defects. ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from 3 independent experiments. Error bars show standard error. n Distribution of different types of phenotypes in embryos at 48 hpf. Data were combined from two independent experiments

Journal: BMC Developmental Biology

Article Title: Clec14a genetically interacts with Etv2 and Vegf signaling during vasculogenesis and angiogenesis in zebrafish

doi: 10.1186/s12861-019-0188-6

Figure Lengend Snippet: Clec14a and C1qr have a partially redundant function during angiogenic sprouting. Kdrl: GFP expression in the trunk region imaged by fluorescent microscopy at 28 hpf ( a - d ), 48 hpf ( e - h ) and 72 hpf ( i - l ). a , e , i Wild-type control embryos; b , f , j clec14a−/− mutants; c , g , k Wild-type embryos injected with 10 ng of c1qr MO; d , h , l clec14a−/− mutant embryos injected with 10 ng of c1qr MO. Note the delayed intersegmental vessel sprouting in clec14a mutants ( b ) and c1qr MO embryos ( c ) at 24 hpf (arrowheads). Partial sprouts (arrowheads) and abnormal vascular connections (arrows) are apparent in the clec14a−/−; c1qr MO embryos ( d , h , l ). m Percentage of embryos with vascular defects. ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from 3 independent experiments. Error bars show standard error. n Distribution of different types of phenotypes in embryos at 48 hpf. Data were combined from two independent experiments

Article Snippet: A translation-blocking c1qr MO (GTCACTCTCATACTACTCGCTTTAG, Gene-Tools Inc), a 5-base c1qr mismatch MO (GTCAgTCTgATAgTACTCggTTTAG), a standard control MO (CCTCTTACCTCAGTTACAATTTATA, Gene-Tools Inc), a previously reported vegfaa MO (GTATCAAATAAACAACCAAGTTCAT) [ ] and etv2 MO2 (CACTGAGTCCTTATTTCACTATATC) [ ] were used for experiments.

Techniques: Expressing, Microscopy, Injection, Mutagenesis

Analysis of vascular marker expression by in situ hybridization at 24 hpf in clec14a−/− embryos injected with 10 ng of c1qr MO. a - d kdrl, e - h fli1a, i - l cdh5, m - p arterial marker cldn5b, q - t venous marker flt4 expression. Note the strong inhibition of angiogenic sprouting in the double clec14a−/− ; c1qr MO embryos (arrowheads). Only weak or partial inhibition of sprouting is observed in clec14a−/− or c1qr MO embryos. u Percentage of embryos with defects in ISV sprouting is greatly increased among c1qr MO; clec14a−/− embryos compared to c1qr MO or clec14a−/− embryos. n refers to the number of embryos analyzed for each marker. * p < 0.05; ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from two independent experiments. Error bars show standard error

Journal: BMC Developmental Biology

Article Title: Clec14a genetically interacts with Etv2 and Vegf signaling during vasculogenesis and angiogenesis in zebrafish

doi: 10.1186/s12861-019-0188-6

Figure Lengend Snippet: Analysis of vascular marker expression by in situ hybridization at 24 hpf in clec14a−/− embryos injected with 10 ng of c1qr MO. a - d kdrl, e - h fli1a, i - l cdh5, m - p arterial marker cldn5b, q - t venous marker flt4 expression. Note the strong inhibition of angiogenic sprouting in the double clec14a−/− ; c1qr MO embryos (arrowheads). Only weak or partial inhibition of sprouting is observed in clec14a−/− or c1qr MO embryos. u Percentage of embryos with defects in ISV sprouting is greatly increased among c1qr MO; clec14a−/− embryos compared to c1qr MO or clec14a−/− embryos. n refers to the number of embryos analyzed for each marker. * p < 0.05; ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from two independent experiments. Error bars show standard error

Article Snippet: A translation-blocking c1qr MO (GTCACTCTCATACTACTCGCTTTAG, Gene-Tools Inc), a 5-base c1qr mismatch MO (GTCAgTCTgATAgTACTCggTTTAG), a standard control MO (CCTCTTACCTCAGTTACAATTTATA, Gene-Tools Inc), a previously reported vegfaa MO (GTATCAAATAAACAACCAAGTTCAT) [ ] and etv2 MO2 (CACTGAGTCCTTATTTCACTATATC) [ ] were used for experiments.

Techniques: Marker, Expressing, In Situ Hybridization, Injection, Inhibition