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Image Search Results
Journal: BMC Developmental Biology
Article Title: Clec14a genetically interacts with Etv2 and Vegf signaling during vasculogenesis and angiogenesis in zebrafish
doi: 10.1186/s12861-019-0188-6
Figure Lengend Snippet: Clec14a and C1qr have a partially redundant function during angiogenic sprouting. Kdrl: GFP expression in the trunk region imaged by fluorescent microscopy at 28 hpf ( a - d ), 48 hpf ( e - h ) and 72 hpf ( i - l ). a , e , i Wild-type control embryos; b , f , j clec14a−/− mutants; c , g , k Wild-type embryos injected with 10 ng of c1qr MO; d , h , l clec14a−/− mutant embryos injected with 10 ng of c1qr MO. Note the delayed intersegmental vessel sprouting in clec14a mutants ( b ) and c1qr MO embryos ( c ) at 24 hpf (arrowheads). Partial sprouts (arrowheads) and abnormal vascular connections (arrows) are apparent in the clec14a−/−; c1qr MO embryos ( d , h , l ). m Percentage of embryos with vascular defects. ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from 3 independent experiments. Error bars show standard error. n Distribution of different types of phenotypes in embryos at 48 hpf. Data were combined from two independent experiments
Article Snippet: A translation-blocking c1qr MO (GTCACTCTCATACTACTCGCTTTAG, Gene-Tools Inc), a
Techniques: Expressing, Microscopy, Injection, Mutagenesis
Journal: BMC Developmental Biology
Article Title: Clec14a genetically interacts with Etv2 and Vegf signaling during vasculogenesis and angiogenesis in zebrafish
doi: 10.1186/s12861-019-0188-6
Figure Lengend Snippet: Analysis of vascular marker expression by in situ hybridization at 24 hpf in clec14a−/− embryos injected with 10 ng of c1qr MO. a - d kdrl, e - h fli1a, i - l cdh5, m - p arterial marker cldn5b, q - t venous marker flt4 expression. Note the strong inhibition of angiogenic sprouting in the double clec14a−/− ; c1qr MO embryos (arrowheads). Only weak or partial inhibition of sprouting is observed in clec14a−/− or c1qr MO embryos. u Percentage of embryos with defects in ISV sprouting is greatly increased among c1qr MO; clec14a−/− embryos compared to c1qr MO or clec14a−/− embryos. n refers to the number of embryos analyzed for each marker. * p < 0.05; ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from two independent experiments. Error bars show standard error
Article Snippet: A translation-blocking c1qr MO (GTCACTCTCATACTACTCGCTTTAG, Gene-Tools Inc), a
Techniques: Marker, Expressing, In Situ Hybridization, Injection, Inhibition